Defined Minimal Culturing: Supports growth and maintenance of Escherichia coli and other enteric bacteria in a chemically defined medium (1.0X M9 Salts, 5.0µg/mL thiamine, 1.0mM MgSO4, 0.1mM CaCl2).

Carbon Source Screening: Evaluate metabolism by supplementing user-defined carbon sources (e.g., glucose, glycerol, acetate, lactose) in a glucose-free baseline.

Auxotroph Complementation: Select and characterize auxotrophic mutants and complementation, leveraging defined nutrients and thiamine supplementation.

Isotopic Labeling: Incorporate 13C/15N labels for metabolomics/proteomics by supplying labeled carbon/nitrogen sources in a minimal background.

Recombinant Protein Expression: Express proteins in Escherichia coli under controlled nutrient conditions to reduce byproducts and standardize growth.

Adaptive Evolution and Kinetics: Perform ALE, growth curves, and yield comparisons under defined Mg2+/Ca2+ conditions.

Antibiotic Selection: Maintain plasmids and perform selection with minimal media to reduce confounding components.

Induction System Tuning: Optimize inductions (e.g., arabinose, lactose) by pairing inducers with chosen carbon sources in glucose-free medium.

Phage Work: Support bacteriophage adsorption/propagation assays that benefit from Mg2+ and Ca2+ availability.

Transport/Chemotaxis Assays: Measure uptake and taxis with defined salts and minimal organics to minimize background signals.