Defined Cultivation of Escherichia coli: Supports growth under precisely defined conditions with glucose, Mg2+, Ca2+, and thiamine.

Metabolic Mutant Selection and Auxotrophy Testing: Differentiates prototrophs from auxotrophs and enables complementation analysis on minimal nutrients.

Carbon Utilization and Catabolite Repression Studies: Evaluates glucose metabolism and regulatory responses in a controlled minimal medium.

Recombinant Protein Expression in Defined Media: Provides consistent growth and reduced byproducts for expression in E. coli.

Quantitative Physiology and Growth Kinetics: Enables reproducible growth curves, yield measurements, and nutrient-limitation studies.

Antibiotic Selection and Plasmid Maintenance: Maintains selectable markers with minimal background components that can confound phenotypes.

Chemotaxis and Motility Assays: Offers a defined matrix with glucose to assess movement toward nutrients.

Biofilm Formation Studies: Supports controlled biofilm assays for Pseudomonas aeruginosa and Escherichia coli on a minimal carbon source.

Induction Optimization for Expression Systems: Facilitates tuning of IPTG or arabinose induction in a nutrient-defined background.