Gel Electrophoresis: Used as the running buffer for Bis-Tris gels (MES buffer) to provide stable pH near 6.0 for high-resolution protein separation.

Protein Purification: Commonly used in cation-exchange chromatography to maintain pH 6.0 during binding and elution steps.

Enzyme Assays: Provides stable buffering near pH 6.0 for acid-range enzyme kinetics and activity measurements.

Cell Culture and Bioprocessing: Buffers mammalian and insect cell media, feeds, and harvest buffers; sterile, endotoxin-tested format supports sensitive workflows.

Viral Vector and Biologics Preparation: Used in formulation, clarification, and purification buffers where low endotoxin and sterile conditions are required.

Buffer Exchange and Dialysis: Serves as a stable pH 6.0 buffer for desalting, diafiltration, and sample conditioning prior to downstream analyses.