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M2101

10X MOPS Buffer. 100mL, Sterile.

Availability

Ships in 1 - 2 Days

Container Size

125mL

Item Count

1 Bottle(s)

Price per Item

$55.00

Fill Volume

100 mL

M2101

10X MOPS Buffer. 100mL, Sterile.

Quantity

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Description
Formulation
Common Applications
Quality Specifications
Storage

10X MOPS Buffer is a concentrated buffering solution used to prepare the running buffer for formaldehyde–agarose RNA gel electrophoresis and for Northern blotting workflows. When diluted to 1X with nuclease-free water, it provides a defined pH environment suitable for separation of RNA under denaturing conditions.

Use at 1X according to protocol for casting and running denaturing agarose gels with formaldehyde, filling electrophoresis chambers, and supporting transfer steps in Northern analysis. Prepare fresh working solutions and discard if contamination or precipitation is observed.

  • MOPS
  • 400 mM
  • Tricine (MW 179.2) 40 mM
  • Iron Sulfate 0.1 mM
  • Ammonium Chloride 95 mM
  • Potassium Sulfate 2.76 mM
  • Calcium Chloride 5 µM
  • Magnesium Chloride 52.5 mM
  • Sodium Chloride 500 mM
  • Ammonium Molybdate 2.92 x 10⁻⁶ mM
  • Boric Acid 4.00 x 10⁻⁴ mM
  • Cobalt Chloride 3.02 x 10⁻⁵ mM
  • Cupric Sulfate 9.62 x 10⁻⁶ mM
  • Manganese Chloride 8.08 x 10⁻⁵ mM
  • Zinc Sulfate 9.74 x 10⁻⁶ mM
  • RNA Agarose Gel Electrophoresis: Used as the running buffer for formaldehyde-denaturing agarose gels and Northern blot workflows with 10X MOPS Buffer.

  • SDS-PAGE (Bis-Tris) Running Buffer: Serves as the running buffer in Bis-Tris protein gels, providing stable near-neutral pH with 10X MOPS Buffer.

  • RNA Handling and Extraction: Maintains RNase-free, stable pH conditions during RNA isolation, cleanup, and storage using 10X MOPS Buffer.

  • Protein Purification: Functions as a buffering component for ion-exchange and size-exclusion chromatography to stabilize proteins with 10X MOPS Buffer.

  • Enzyme Assays: Provides consistent pH near physiological conditions for enzymatic activity and kinetics with 10X MOPS Buffer.

  • MOPS Minimal Medium Preparation: Buffers defined media for growth and physiology studies of Escherichia coli and other bacteria using 10X MOPS Buffer.

  • Sample Dialysis and Preparation: Maintains target pH during dialysis and buffer exchange steps with 10X MOPS Buffer.

  • General Laboratory Buffering: Stabilizes pH in biochemical workflows where phosphate or Tris buffers are unsuitable, employing 10X MOPS Buffer.

  • pH: 7.40 ± 0.04
Storage Temperature
Frozen
Shelf Life
730 days
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