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M1215

M9 Minimal Salts Agar Plates with Uracil-250. 100mm, 20 Plates per Sleeve, Sterile.

Availability

Ships in 6 - 14 Days

Container Size

100mm

Item Count

20 Plate(s)

Price per Item

$102.00

Fill Volume

25 mL

COA
COA

M1215

M9 Minimal Salts Agar Plates with Uracil-250. 100mm, 20 Plates per Sleeve, Sterile.

Quantity

Minimum is set to 4 for this product*

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Description
Formulation
Common Applications
Storage

M9 Minimal Salts Agar Plates with Uracil-250 is a defined minimal agar medium for cultivation and analysis of bacteria such as E. coli under controlled nutrient conditions. Glucose serves as the primary carbon and energy source, thiamine supplies vitamin B1, and magnesium and calcium provide essential divalent cations. The addition of uracil (250 µg/mL) supports the growth of uracil-auxotrophic strains on a solid minimal matrix.

  • 1X M9 Salts
  • 1.0% Cerelose Anhydrous Dextrose (Glucose)
  • 1.0mM Thiamine
  • 0.2mM Magnesium Sulfate
  • 0.1mM Calcium Chloride
  • 1.5% Agar
  • 250µg/mL Uracil

  • Growth of Uracil Auxotrophs: Supports maintenance and propagation of pyrE/pyrF mutants of Escherichia coli and related enterics with 250µg/mL uracil.

  • Defined Minimal Culture: Enables controlled growth of E. coli on 1X M9 salts with 1.0% glucose for physiology and metabolism studies.

  • Auxotrophy-Based Selection/Complementation: Facilitates selection and verification of pyrimidine biosynthesis mutants and complemented strains on minimal medium.

  • Bacteriophage Plaque Assays: Suitable for phage work (e.g., P1, T4, λ) on E. coli with 0.2mM MgSO₄ and 0.1mM CaCl₂ enhancing adsorption.

  • Carbon Utilization and CCR Assays: Tests glucose utilization and catabolite repression under defined 1.0% glucose conditions.

  • Recombineering and Genome Editing Workflows: Enables selection schemes involving pyrF/pyrE counterselection complemented by uracil.

  • Antibiotic Susceptibility on Minimal Media: Assesses drug responses of E. coli and Salmonella enterica under defined nutrient conditions.

  • Mutation Rate and Synthetic Lethality Screens: Identifies genetic interactions that manifest under minimal, glucose-defined conditions with required vitamins.

  • Expression and Metabolic Flux Studies: Minimizes complex nutrient background for evaluating gene expression and metabolic pathway activity.

Storage Temperature
Room Temperature
Shelf Life
300 days
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