Selection of Kanamycin-Resistant Transformants: Isolation and maintenance of E. coli clones carrying kanamycin-resistant plasmids at 50 µg/mL.

Recombinant Colony Screening: Post-transformation colony picking and verification under kanamycin selection.

Conjugation/Transduction Selection: Recovery of kanamycin-marked alleles or transposon insertions in E. coli.

CRISPR/Cas Editing Selection: Enrichment of edited E. coli clones using kanamycin resistance markers.

Defined Minimal Growth Studies: Metabolism, auxotrophy, and fitness assays on M9 with glucose as the sole carbon source.

Expression Studies in Minimal Media: Controlled background for gene expression from kanamycin-selectable constructs.

Plasmid Stability Assays: Monitoring retention of kanamycin-selectable plasmids under continuous selection.

Colony Purification and Storage: Streaking and short-term storage of kanamycin-resistant E. coli strains on 1.5% agar.

Antibiotic Marker Verification: Confirmation of kanamycin resistance phenotypes in laboratory strains.