Selection of Auxotrophs/Prototrophs: Defined 1X M9 with 1.0% glucose and thiamine enables genetic selection for biosynthetic capabilities in E. coli and related bacteria.

Metabolic and Carbon Utilization Studies: Glucose-based minimal agar supports phenotyping of central carbon metabolism and substrate utilization in E. coli.

Antibiotic Selection on Defined Medium: Maintains controlled ionic conditions (Mg2+, Ca2+) for plasmid maintenance and selection with antibiotics in E. coli.

Replica Plating for Nutritional Requirements: Used to map auxotrophies and screen revertants or suppressors on minimal plates.

Bacteriophage Assays (Base Plate): Serves as the bottom agar for soft-agar overlays in phage plaque assays with E. coli and coliphages.

CFU Enumeration and Colony Isolation: Supports counting and isolating colonies of organisms adapted to minimal media, including E. coli.

Conjugation and Transduction Selections: Facilitates selection of recombinants using auxotrophic markers during Hfr conjugation or P1 transduction in E. coli.

Defined-Medium Culturing for Systems Biology: Provides reproducible growth conditions for transcriptomics/proteomics of E. coli under minimal nutrient regimes.

Growth of Non-Fastidious Model Bacteria: Supports cultivation of minimal-medium-competent strains such as E. coli and some Pseudomonas spp.