Isolation of Enteric Gram-Negatives: Selective recovery of Gram-negative rods from clinical specimens (urine, stool, wound) while suppressing Gram-positives.

Differentiation by Lactose Fermentation: Distinguishes lactose fermenters (pink/red colonies) such as Escherichia coli, Klebsiella, and Enterobacter from non-fermenters (colorless) like Salmonella, Shigella, and Pseudomonas aeruginosa.

Coliform Detection: Screening and enumeration of coliforms in water, food, and dairy testing based on lactose fermentation and bile tolerance.

Clinical Microbiology Workflows: Primary plating and subculture to guide identification and downstream biochemical or MALDI-TOF testing of Enterobacterales.

Public Health Surveillance: Recovery of enteric pathogens (e.g., non-lactose-fermenting Salmonella and Shigella) from stool and enrichment broths.

Environmental Monitoring: Detection of Gram-negative contaminants and coliforms in pharmaceutical and cleanroom surveillance programs.

Mixed Culture Isolation: Selective plating from polymicrobial specimens to reduce overgrowth by Gram-positives and fastidious flora.

Quality Control and Validation: Routine QC with reference strains to verify selective and differential performance for lactose fermentation and bile tolerance.

Food and Beverage Testing: Monitoring of hygiene indicators and potential enteric contaminants in production environments.