Selective Growth of KanR Strains: Enriches kanamycin-resistant Escherichia coli using LB (1.0% Tryptone, 0.5% Yeast Extract, 1.0% NaCl) with 50µg/mL Kanamycin.

Plasmid Propagation: Grows kanamycin-marked plasmids in overnight cultures for miniprep/midiprep workflows.

Colony Picking and Screening: 3 mL tube cultures for rapid screening of transformants under kanamycin selection.

Protein Expression: Maintains selection during recombinant protein production in kanR Escherichia coli expression strains.

Glycerol Stock Preparation: Produces long-term stocks from kanamycin-selected cultures to preserve plasmid integrity.

Library Maintenance: Sustains kanamycin-selected genomic or plasmid libraries in Escherichia coli.

Transformant Verification: Confirms antibiotic resistance and insert presence via selective liquid culture growth.

Cloning Workflows: Supports subcloning, ligation, and assembly products carrying kanR markers through selective growth.