Antibiotic Selection in Liquid Culture: Selects and maintains plasmid-bearing E. coli (AmpR/CarbR) at 100 µg/mL carbenicillin.

Plasmid DNA Propagation: Grows high-copy and low-copy plasmids in E. coli for mini/midi/maxi preps.

Overnight Starter Cultures: Generates selective starters for cloning, sequencing, and downstream expression.

Recombinant Protein Expression: Cultures E. coli harboring beta-lactamase–selectable expression vectors prior to induction.

Strain and Library Maintenance: Preserves engineered strains and pooled plasmid libraries in E. coli under selection.

Glycerol Stock Preparation: Produces cryostocks of plasmid-bearing E. coli while maintaining selective pressure.

Scale-Up/Inoculation: Inoculates larger baffled-flask or bioreactor cultures from selective starters.

Growth Curves and QC: Performs OD600 growth analyses and antibiotic stability checks in selective LB.

Alternative Gram-Negative Cultivation: Propagates carbenicillin-selectable vectors in organisms such as Agrobacterium tumefaciens, when appropriate.