Selection and propagation of Zeocin-resistant plasmids: Grows E. coli carrying Sh ble (Zeocin) resistance at 25 µg/mL for reliable selection.

Plasmid amplification for cloning: Produces high-yield overnight cultures of Zeocin-marked vectors for mini/midi/maxi preps.

Recombinant protein expression: Supports expression in E. coli from vectors using Zeocin as the selectable marker.

Post-transformation outgrowth and selection: Enables recovery and enrichment of Zeocin-resistant transformants in liquid culture.

Starter culture and inoculum preparation: Generates consistent inocula for downstream fermentations or plate streaking under Zeocin selection.

Glycerol stock creation: Preserves Zeocin-resistant E. coli strains for long-term storage.

Bacterial amplification of shuttle vectors: Maintains Zeocin-marked plasmids in E. coli prior to use in other host systems.