Selective Cultivation: Growth and maintenance of kanamycin-resistant plasmid-bearing E. coli in liquid culture (50 µg/mL kanamycin sulfate).

Cloning and Plasmid Propagation: Expansion of transformants for plasmid DNA preparation (mini/midi/maxi preps) in E. coli under kanamycin selection.

Protein Expression: Culturing recombinant E. coli harboring kanamycin-selectable expression vectors for small- to large-scale protein production.

Library Maintenance: Preservation and amplification of kanamycin-marked cloning libraries or pooled transformant populations in E. coli.

Glycerol Stock Preparation: Long-term storage of kanamycin-resistant E. coli strains under selective pressure.

Strain Validation: Verification of kanamycin resistance markers, insertion cassettes, and construct integrity in engineered E. coli strains.

Conjugation/Genetic Transfer Selection: Post-mating selection of recipients or transconjugants carrying kanamycin resistance in E. coli and related enteric bacteria.

Growth and Fitness Assays: Comparative growth curves and stability testing of kanamycin-marked strains in LB with selection.