Selective Cultivation of Kanamycin-Resistant E. coli: Growth and maintenance of plasmid-bearing strains under 50 µg/mL kanamycin selection.

Plasmid Selection and Amplification: Enrichment of transformants and propagation of kanamycin-marked plasmids for DNA prep workflows.

Starter and Overnight Cultures for Protein Expression: Preparation of selective cultures of recombinant E. coli for downstream induction.

Liquid Culture Screening of Colonies: Picking colonies into selective broth for verification by PCR, restriction analysis, or sequencing.

Library Maintenance: Culturing and passaging of kanamycin-marked plasmid or genomic libraries.

Glycerol Stock Preparation: Long-term storage of kanamycin-resistant E. coli strains grown in selective media.

Selective Growth in Automated or Scale-Up Systems: Use in shake flasks and bioreactors where kanamycin selection is required.