Kanamycin-Resistant Plasmid Selection: Selects and maintains transformants of Escherichia coli carrying kanamycin-resistance plasmids using 25µg/mL kanamycin sulfate.

Routine Cultivation of Resistant Strains: Grows overnight and starter cultures of kanamycin-resistant E. coli and other Gram-negative bacteria.

Recombinant Protein Expression: Supports expression workflows in kan-resistant E. coli strains prior to downstream purification.

Cloning and Strain Construction: Enables recovery and outgrowth after transformation, ligation, or Gibson/Golden Gate assembly under kanamycin selection.

Colony Picking and Plasmid Preparation: Expands colonies for miniprep/midiprep with consistent kanamycin selection pressure.

Glycerol Stock Preparation: Produces long-term frozen stocks of kanamycin-resistant bacterial strains.

Library Maintenance: Maintains pooled mutant or plasmid libraries bearing kanamycin-selectable markers.

Agrobacterium Workflows: Cultivates Agrobacterium tumefaciens carrying kan-selectable binary vectors for plant transformation.

Conjugation and Mating Selections: Selects transconjugants in inter- or intra-species matings involving kanamycin resistance markers.

Starter Culture Preparation for Fermentation: Generates kan-selected seed cultures for scale-up in bioprocessing.