Selective Cultivation of Carbenicillin-Resistant Escherichia coli: Growth of E. coli harboring bla/ampR plasmids using 50 µg/mL carbenicillin for selection.

Plasmid Maintenance and Amplification: Sustains selection pressure to maintain beta-lactamase plasmids for high-yield DNA prep.

Overnight Cultures for Molecular Cloning: Produces dense cultures for miniprep/midiprep workflows.

Starter and Seed Cultures for Protein Expression: Inoculation of expression media with carbenicillin-selected E. coli strains.

Post-Transformation Recovery and Outgrowth: Enrichment of transformants prior to plating or downstream assays.

Liquid Screening of Clones: Small-scale selective cultures to verify insert presence and stability.

Glycerol Stock Preparation: Long-term storage of carbenicillin-resistant E. coli strains.

Animal-Free Microbial Workflows: Supports applications requiring animal-free components via 1.69% animal-free Soytone and 0.50% yeast extract.

General Bacterial Cultivation: Standard LB base (1.00% NaCl) for routine growth of engineered E. coli under selection.

Pre-Fermentation Scale-Up: Selective expansion of recombinant E. coli prior to larger-scale bioprocessing.