Selective Cultivation: Growth of ampicillin-resistant E. coli carrying bla/ampR plasmids under antibiotic selection.

Plasmid Selection and Maintenance: Sustains selective pressure to retain target plasmids during routine culturing.

Overnight Cultures for DNA Prep: Produces high-density cultures for plasmid miniprep/midiprep/maxiprep workflows.

Transformation Recovery and Selection: Post-transformation outgrowth under ampicillin to enrich for successful transformants.

Starter Cultures for Protein Expression: Inoculation and scale-up of ampicillin-resistant recombinant E. coli expression strains.

Glycerol Stock Preparation: Long-term storage of validated ampicillin-resistant clones.

Colony Picking into Broth: Rapid liquid screening of colonies for insert verification under selection.

Growth Curve and OD600 Measurements: Monitoring bacterial growth kinetics while maintaining plasmid selection.

Library Propagation: Amplification of plasmid libraries and variant pools in selective liquid culture.