Selective Culture of Ampicillin-Resistant Bacteria: Growth and maintenance of AmpR Escherichia coli strains using 50 µg/mL ampicillin.

Plasmid Propagation and DNA Preparation: Amplification of plasmids for mini/midi/maxi-preps from AmpR E. coli cultures.

Cloning Workflows: Culture of ligation, Gibson, or Golden Gate assemblies under ampicillin selection.

Transformation and Selection: Post-recovery selection of transformed AmpR cells and inoculation of liquid selective cultures.

Protein Expression: Growth of AmpR expression hosts (e.g., T7-based systems) for recombinant protein production in E. coli.

Starter Cultures and Scale-Up: Overnight cultures for scaling to larger volumes while maintaining ampicillin selection.

Glycerol Stock Preparation: Preparation of long-term frozen stocks from AmpR strains.

Library Construction and Screening: Maintenance and selection of plasmid-based libraries carrying ampicillin resistance markers.

Colony Verification Workflows: Culturing candidate clones for downstream PCR, restriction digest, or sequencing validation.

Plasmid Stability Assurance: Continuous selection to minimize plasmid loss during routine subculture of AmpR E. coli.