Routine Bacterial Cultivation: General growth and propagation of Escherichia coli using the standard LB Miller formulation (1.0% Tryptone, 0.5% Yeast Extract, 1.0% NaCl).

Plasmid Cloning and Maintenance: Culturing recombinant Escherichia coli for plasmid construction, maintenance, and selection with antibiotics.

Transformation Recovery/Outgrowth: Post-heat shock or electroporation recovery of Escherichia coli prior to plating or selection.

Starter Cultures for DNA Prep: Overnight cultures of Escherichia coli for miniprep, midiprep, and maxiprep workflows.

Protein Expression Cultures: Growth of recombinant Escherichia coli for IPTG- or autoinduction-based protein expression.

Antibiotic Selection Studies: Assessing plasmid retention and expression in Escherichia coli under selective pressure.

Glycerol Stock Preparation: Growing Escherichia coli prior to long-term cryopreservation in glycerol.

Bacterial Library Amplification: Expansion of cloned libraries in Escherichia coli for downstream screening.

Conjugation/Mating Assays: Liquid co-culture support for plasmid transfer between Escherichia coli strains.

Phage Work (Liquid Steps): Culturing host Escherichia coli strains for bacteriophage amplification steps prior to plating on LB agar.