Antibiotic Selection of Transformants: Selects kanamycin-resistant colonies (e.g., plasmid-bearing Escherichia coli) during cloning and synthetic biology.

Plasmid Maintenance and Expression: Maintains strains carrying kanamycin-selectable plasmids for protein expression and functional studies.

Colony Screening for Downstream Workflows: Enables picking colonies for PCR, sequencing, or expression verification under kanamycin selection.

Genetic Engineering and Recombineering: Selects recombinants using kanamycin resistance markers in genome editing workflows.

Bacterial Conjugation and Transposon Mutagenesis: Recovers transconjugants or insertion mutants carrying kanamycin resistance.

Strain Preservation and Archiving: Supports streaking and maintenance of kanamycin-selected strains for short- and medium-term storage.

Reporter and Construct Validation: Screens colonies expressing fluorescent or enzymatic reporters linked to kanamycin resistance.

Educational and QC Applications: Demonstrates antibiotic selection on LB (1.0% Tryptone, 0.5% Yeast Extract, 1.0% Sodium Chloride, 1.5% Agar) with 50µg/mL Kanamycin Sulfate; verifies performance of kanamycin resistance cassettes.

Library and Metagenomic Selections: Plates combinatorial or environmental DNA libraries to enrich clones carrying kanamycin-selectable vectors.

Host Range Assessments: Tests compatibility of kanamycin-selectable constructs across laboratory strains such as Escherichia coli.