Ampicillin-Resistant Transformant Selection: Isolates colonies of Escherichia coli harboring AmpR (bla) plasmids using 100 µg/mL ampicillin.

Plasmid Cloning and Maintenance: Maintains AmpR plasmids in E. coli using LB Luria (1.00% Tryptone, 0.50% Yeast Extract, 0.05% NaCl, 1.50% Agar) with ampicillin.

Colony Picking for Minipreps/Sequencing: Provides well-isolated AmpR colonies for downstream plasmid prep and sequence verification.

Blue-White Screening (With X-gal/IPTG): Enables α-complementation screening of AmpR plasmids in E. coli when supplemented.

Transformation Efficiency and CFU Enumeration: Quantifies transformant yield on selective medium containing 100 µg/mL ampicillin.

Streaking for Single Colonies: Purifies clonal AmpR E. coli isolates and supports strain maintenance.

Expression Construct Verification: Screens AmpR expression plasmids in E. coli prior to liquid culture induction.

Control for Antibiotic Selection: Differentiates AmpR versus Amp-sensitive E. coli strains by growth/no-growth.

Glycerol Stock Starter Colonies: Provides clean AmpR colonies for long-term archival of E. coli strains.