Transformation and Clone Selection: Selects kanamycin-resistant transformants, commonly in E. coli and Agrobacterium tumefaciens.

Plasmid Maintenance: Maintains plasmids carrying kanamycin resistance markers during routine culturing of E. coli.

Colony Isolation and Purification: Streaking and single-colony isolation under kanamycin selection to ensure clonal purity.

CRISPR/Cas Genome Editing: Enriches edited clones harboring kanamycin-selectable repair or editing cassettes.

Conjugation and Mating Selection: Recovers kanamycin-resistant transconjugants after bacterial mating (e.g., donor to Agrobacterium tumefaciens or E. coli recipients).

Transposon Mutagenesis: Selects insertion mutants when the transposon carries a kanamycin resistance cassette.

Library Construction and Screening: Builds and maintains kanamycin-selected genomic or plasmid libraries in E. coli.

Blue-White Screening (with Kan Vectors): Alpha-complementation on kanamycin plates supplemented with X-gal/IPTG to identify inserts.

Strain Storage and Stability Testing: Evaluates retention of kanamycin-marked plasmids/constructs during passaging or storage.

Work with Alternative Hosts: Selection in other kanamycin-susceptible hosts such as Bacillus subtilis (vector-dependent).