Blue/White Colony Screening: Alpha-complementation of lacZ using IPTG (40 µg/mL) and Bluo-Gal (100 µg/mL) for rapid identification of recombinant clones in E. coli.

Tri-Antibiotic Selection: Concurrent selection for plasmids bearing carbenicillin (100 µg/mL), kanamycin (50 µg/mL), and tetracycline (10 µg/mL) resistance markers in E. coli.

Multi-Plasmid Maintenance: Stable co-maintenance of compatible vectors with distinct resistance cassettes in cloning and expression strains of E. coli.

Library Construction and Screening: High-throughput colony picking and screening of ligation, Gibson, or Golden Gate libraries under triple selection in E. coli.

Promoter/Reporter Validation: IPTG-inducible reporter assays (lac-based) while maintaining antibiotic selection in E. coli.

Replica Plating and Phenotype Confirmation: Verification of transformants and plasmid combinations via tri-antibiotic replica plating in E. coli.

Conjugation/Mobilization Workflows: Selection of donor, recipient, and helper plasmids post-mating when multiple resistance markers are required in E. coli.

CRISPR/Cas Plasmid Workflows: Co-selection of editing and helper plasmids with distinct resistance markers in E. coli cloning strains.