Selection of Kanamycin-Resistant Transformants: Isolates colonies carrying kanamycin-resistance plasmids on LB agar with 50 µg/mL kanamycin sulfate.

Plasmid Maintenance in Escherichia coli: Preserves kan-marked plasmids during routine growth and passaging on 1.0% tryptone/0.5% yeast extract/1.0% NaCl/1.5% agar plates.

Colony Screening for Cloning: Enables picking colonies for PCR, restriction analysis, or sequencing to verify inserts from kan-selective plates.

CFU Enumeration: Performs titering and viability counts of kan-resistant bacterial cultures.

CRISPR/Cas Editing Selection: Selects edited clones carrying kanamycin cassettes or helper plasmids.

Conjugation/Transduction Selection: Recovers recipients that acquired kan-marked elements after mating or phage-mediated transfer.

Protein Expression Workflows: Maintains expression strains harboring kan-resistant expression vectors prior to induction.

Blue-White Screening (with Supplements): Supports alpha-complementation when supplemented with IPTG/X-Gal on kan-selective LB agar.

Strain Purification and Archiving: Streaks for single colonies and maintains culture purity under kan selection.

Agrobacterium and Other Bacteria: Selects kan-resistant plasmids in organisms such as Agrobacterium tumefaciens and other kan-susceptible bacteria.