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L7204

LB Agar Plates with Ampicillin-100. 100mm, 20 Plates per Sleeve, Sterile. (Heat Shrink)

Availability

Ships in 6 - 14 Days

Container Size

100mm

Item Count

20 Plate(s)

Price per Item

$95.00

Fill Volume

25 mL

L7204

LB Agar Plates with Ampicillin-100. 100mm, 20 Plates per Sleeve, Sterile. (Heat Shrink)

Quantity

Minimum is set to 8 for this product*

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Description
Formulation
Common Applications
Storage

LB Agar Plates with Ampicillin-100 are nutrient-rich agar plates based on LB medium and supplemented with 100 µg/mL ampicillin sodium salt. This medium supports the growth and selection of ampicillin-resistant E. coli strains and facilitates maintenance of plasmid-bearing transformants.

  • 1.0% Tryptone
  • 0.5% Yeast Extract
  • 1.0% Sodium Chloride
  • 1.5% Agar
  • 100µg/mL Ampicillin Sodium Salt
  • Selection of Ampicillin-Resistant Transformants: Isolates colonies carrying AmpR plasmids in E. coli after transformation.

  • Plasmid Cloning and Maintenance: Maintains selective pressure for plasmid-bearing E. coli during routine subculture.

  • Blue-White Screening: Enables lacZ-based clone screening in E. coli when supplemented with X-Gal/IPTG.

  • Colony Isolation and Purification: Streaks for single colonies to obtain clonal AmpR E. coli populations.

  • Library Construction and Screening: Selects recombinant libraries and facilitates replica plating under ampicillin selection in E. coli.

  • Protein Expression Plasmid Selection: Maintains expression vectors with AmpR in production or induction strains of E. coli.

  • Competent Cell QC: Verifies transformation efficiency and antibiotic selection fidelity in E. coli.

  • Miniprep/Maxiprep Pre-culture Colony Picking: Sources verified AmpR colonies for downstream plasmid DNA preparation from E. coli.

Storage Temperature
Refrigerated
Shelf Life
60 days
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