Plasmid Cloning and Selection: Selects E. coli transformants carrying Sh ble (zeoR) plasmids on LB Lennox agar with 50µg/mL Zeocin®.

Bacterial Transformation Screening: Enables rapid colony picking of zeocin-resistant E. coli after heat-shock or electroporation.

Plasmid Maintenance and Stability: Maintains selection pressure for zeoR plasmids in E. coli to prevent plasmid loss.

Genome Editing and Recombineering: Screens E. coli edits using zeocin-selectable cassettes during CRISPR/recombineering workflows.

Library Construction and Enrichment: Selects pooled plasmid libraries (e.g., mutagenesis or sgRNA) in E. coli under Zeocin® pressure.

Colony Isolation and CFU Enumeration: Isolates single colonies and quantifies viable zeocin-resistant E. coli CFUs.

Bacterial Conjugation/Mating Selection: Selects donor/recipient E. coli transconjugants carrying zeoR vectors.

Protein Expression Vector Selection: Maintains zeoR expression plasmids in E. coli prior to induction in liquid culture.

Quality Control of Resistance Cassettes: Verifies functionality of Sh ble cassettes and Zeocin® working concentration on LB Lennox agar.

Strain Verification and Archiving: Confirms genotype/plasmid presence and streaks zeoR E. coli for long-term plate stocks.