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L7010

LB Agar Plates with Carbenicillin-100. 100mm, 20 Plates per Sleeve, Sterile. (Heat Shrink)

Availability

Ships in 1 - 2 Days

Container Size

100mm

Item Count

20 Plate(s)

Price per Item

$100.00

Fill Volume

25 mL

L7010

LB Agar Plates with Carbenicillin-100. 100mm, 20 Plates per Sleeve, Sterile. (Heat Shrink)

Quantity

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Description
Formulation
Common Applications
Storage

LB Agar Plates with Carbenicillin-100 are solid media used for growth and selection of carbenicillin-resistant E. coli strains.

The inclusion of carbenicillin disodium salt at 100 µg/mL enables selection of plasmid-bearing transformants and facilitates maintenance of carbenicillin-selectable plasmids.

  • 1.0% Tryptone
  • 0.5% Yeast Extract
  • 1.0% Sodium Chloride
  • 1.5% Agar
  • 100µg/mL Carbenicillin Disodium Salt
  • Plasmid Selection and Maintenance: Selects and stabilizes bla (AmpR) plasmids in Escherichia coli, with reduced satellite colony formation versus ampicillin.

  • Bacterial Transformation Screening: Recovers and identifies transformants after heat-shock or electroporation on 100 µg/mL carbenicillin.

  • Colony Picking and Expansion: Isolates single AmpR colonies for minipreps, sequencing, and strain propagation.

  • Blue-White Screening: Supports lacZ α-complementation with X-gal/IPTG to distinguish recombinants on AmpR background.

  • Protein Expression Workflows: Maintains AmpR expression vectors in Escherichia coli prior to induction and scale-up.

  • CRISPR/Genome Editing Cloning: Selects gRNA/Cas plasmids in Escherichia coli during construct assembly and verification.

  • Library Construction and Maintenance: Propagates cDNA, gRNA, or mutant libraries carried on AmpR plasmids.

  • Glycerol Stock Preparation: Generates long-term stocks from verified AmpR colonies for reproducible workflows.

Storage Temperature
Refrigerated
Shelf Life
90 days
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