Plasmid Selection in Escherichia coli: Selects and maintains bla/ampR plasmid-bearing transformants using 50 µg/mL carbenicillin; minimizes satellite colonies compared to ampicillin.

Colony Screening and Picking: Plates cloning reactions and transformations to isolate single colonies for downstream analysis.

Blue-White Screening: When supplemented with X-gal/IPTG, supports lacZ α-complementation for insert screening in E. coli.

Library Construction and Screening: Plating and selection of combinatorial, mutagenesis, or genomic libraries carrying bla-marked vectors.

CFU Enumeration and Transformation Efficiency: Quantifies colony-forming units and transformation efficiency of competent E. coli.

Strain Maintenance and Archiving: Streaking and short-term storage of carbenicillin-resistant E. coli strains on solid LB.

Conjugation/Mating Selection: Selects transconjugants carrying beta-lactamase plasmids after bacterial mating assays.

CRISPR/Genome Editing Workflows: Screens edited clones maintained on bla-selectable cloning vectors in E. coli.

Phagemid/Cloning Vector Recovery: Plating of ligations or phagemid rescue workflows using bla/ampR vectors.

Starter Culture Initiation for Expression: Picking verified colonies into liquid media for small-scale expression prescreens.