Antibiotic Selection of Transformants: Selects kanamycin-resistant colonies (e.g., plasmid-bearing E. coli) after transformation.

Colony Isolation and Purification: Streaking for single colonies under kanamycin to obtain clonal, plasmid-maintained isolates.

Plasmid Maintenance and Propagation: Routine subculture of strains carrying kanamycin-selectable plasmids to preserve selection.

Colony PCR/Screening: Picking kanamycin-resistant colonies for PCR, restriction analysis, or sequencing-based clone verification.

Transformation Efficiency and CFU Enumeration: Plating to quantify viable kanamycin-resistant transformants per μg DNA.

Genetic Engineering Selection: Selection of recombineering, CRISPR/Cas9 edits, or insertion events marked by kanamycin resistance in E. coli.

Conjugation/Transduction Assays: Selecting kanamycin-marked transconjugants or transductants in bacterial genetics workflows.

Library Plating and Screening: Amplification and screening of plasmid or mutagenesis libraries carrying kanamycin resistance.

Replica Plating and Stability Testing: Assessing plasmid retention and fitness under kanamycin selection pressure.

Strain Stocking and Short-Term Storage: Maintaining selective plate stocks of engineered E. coli strains.