Plasmid Selection and Maintenance: Selects for bla (AmpR/CarbR) plasmids in Escherichia coli, with reduced satellite colonies versus ampicillin.

Bacterial Transformation and Cloning: Plates heat-shock or electroporation transformants for recovery and screening of recombinant clones.

Blue-White Screening: When supplemented with X-gal/IPTG, enables lacZα-complementation screening of recombinant colonies.

Colony Screening (PCR/Sanger Prep): Grows isolated colonies for colony PCR, plasmid minipreps, and sequencing verification.

CFU Enumeration Under Selection: Quantifies transformant efficiency or library diversity while maintaining antibiotic pressure.

CRISPR and Library Workflows: Selects edited cells or pooled libraries carrying beta-lactamase-marked vectors in Escherichia coli.

Conjugation/Mating Selection: Recovers and selects transconjugants harboring beta-lactamase plasmids on selective plates.

Expression Strain Maintenance: Maintains inducible or constitutive expression plasmids with beta-lactamase markers in Escherichia coli.

Strain Banking and Picking: Isolates single colonies for starter cultures and glycerol stock preparation under selection.

Plasmid Stability Assays: Monitors segregational stability and retention of bla-marked plasmids across passages.