Antibiotic Selection of Transformants: Selects kanamycin-resistant colonies of Escherichia coli carrying kanR plasmids (50 µg/mL kanamycin).

Cloning and Plasmid Maintenance: Maintains recombinant constructs with kanamycin resistance markers through subculturing on selective plates.

Streaking for Isolation and Purity: Isolates single colonies under kanamycin selection to verify strain purity.

Colony Picking and Screening: Supports colony PCR, restriction analysis, or sequencing workflows by picking from selective colonies.

Transformation Efficiency and CFU Enumeration: Quantifies transformants and determines efficiency on kanamycin-selective media.

Replica Plating and Library Screening: Uses the 245×245 mm format for high-density arraying and selection of mutant or plasmid libraries.

Conjugation/Mating Selection: Selects transconjugants carrying kanR markers after bacterial mating experiments.

Strain Verification: Confirms retention of kanR plasmids in E. coli by growth on selective LB agar.

Short-Term Strain Maintenance: Stores kanamycin-resistant colonies at 4°C on plates for near-term experiments.