Antibiotic Selection of Plasmid-Bearing Clones: Selects E. coli carrying bla/ampR plasmids using 100 µg/ml carbenicillin for robust colony growth.

Bacterial Transformation and Cloning: Plates heat-shock or electroporated E. coli to recover and select transformants.

Colony Screening (Colony PCR/Sanger Prep): Grows discrete colonies for downstream PCR, minipreps, and sequencing.

Blue-White Screening: When supplemented with X-Gal/IPTG, enables lacZ α-complementation screening on carbenicillin selection.

Strain Maintenance and Streaking: Maintains selection pressure for plasmid retention during routine passaging of E. coli.

Plasmid Library Propagation: Supports high-complexity library plating and colony picking on 245 mm low-profile plates.

Recombinant Expression Prescreening: Picks verified carbenicillin-resistant colonies for small-scale expression tests.

Glycerol Stock Preparation: Isolates single resistant colonies for stable long-term strain archiving.

Conjugation/Mating with Selection: Recovers transconjugants in E. coli using carbenicillin-selectable plasmids.

Reduction of Satellite Colonies: Uses carbenicillin to minimize satellites compared to ampicillin for cleaner selection.