Antibiotic Selection of Transformants: Isolates kanamycin-resistant colonies after transformation of Escherichia coli with KanR plasmids (50 µg/mL kanamycin).

Plasmid Maintenance: Maintains selection pressure for KanR vectors during routine subculture and storage of E. coli strains.

Colony Isolation and Purification: Streaks for single colonies on LB agar (1.0% tryptone, 0.5% yeast extract, 1.0% NaCl, 1.5% agar) to obtain clonal KanR isolates.

Colony PCR Screening: Screens KanR transformants by colony PCR to verify inserts or constructs.

Expression Strain Selection: Selects and maintains KanR expression plasmids in protein production hosts (e.g., E. coli BL21 derivatives).

CFU Enumeration and Titering: Quantifies kanamycin-resistant cells after transformation, electroporation, or recovery.

Replica Plating: Confirms antibiotic resistance and genotype by transferring colonies onto selective LB-Kan plates.

Conjugation/Transposon Selection: Selects transconjugants or transposon insertions carrying KanR markers in enteric bacteria (e.g., E. coli, Salmonella enterica).

Library Construction and Screening: Selects plasmid or genomic libraries with KanR backbones for downstream analysis.

High-Density Colony Arrays: Uses 245x245 mm low-profile plates for gridding, robotic colony picking, and large-scale screens on selective media.