Selection of Transformants: Recovery and selection of Escherichia coli transformants carrying bla/ampR plasmids using 150 µg/mL carbenicillin.

Plasmid Maintenance: Propagation and stability of beta-lactamase–marked cloning/expression vectors in E. coli.

Colony Isolation and Purification: Streaking and restreaking to obtain pure carbenicillin-resistant colonies.

Colony PCR and Screening: Screening recombinant E. coli colonies for inserts directly from selective plates.

Transformation Efficiency and CFU Counting: Quantifying competent cell performance on selective LB agar.

High-Density and Replica Plating: Library screening and replica plating on 245 mm plates for large-format workflows.

Blue-White Screening (With Supplements): Alpha-complementation screening when plates are supplemented with X-gal/IPTG.

Conjugation/Mating Selection: Selecting recipients transconjugants acquiring bla-marked plasmids in E. coli and related Gram-negatives.

Colony Picking for Downstream Work: Selecting clones for minipreps, glycerol stocks, and expression studies.

Strain Maintenance: Routine culture of carbenicillin-resistant laboratory E. coli strains.