Selection of Spectinomycin-Resistant Transformants: Isolates colonies harboring spec/aadA-marked plasmids in Escherichia coli at 100 µg/mL spectinomycin.

Plasmid Maintenance and Propagation: Maintains selection pressure for spec-resistant cloning and expression vectors in E. coli.

Colony Screening and Picking: Supports rapid screening of recombinant colonies post-transformation without additional inducers or indicators.

CFU Enumeration Under Selection: Quantifies viable spectinomycin-resistant cells after transformations, conjugations, or electroporations.

Conjugation and Transconjugant Selection: Selects spectinomycin-marked transconjugants in bacteria such as Escherichia coli and Agrobacterium tumefaciens.

Transposon Mutagenesis Screens: Recovers insertion mutants carrying spectinomycin resistance cassettes.

Gene Knockout/Replacement Selection: Selects chromosomal edits introduced with spectinomycin resistance markers.

Strain Preservation and Routine Streaking: Maintains engineered strains on solid LB Lennox with continued spectinomycin selection.

Library Construction and Maintenance: Supports maintenance of spec-selected plasmid or genomic libraries in E. coli.

Quality Control of Constructs: Verifies retention of spec-marked vectors during subcloning and propagation workflows.