Antibiotic Selection of Transformants: Isolation of kanamycin-resistant colonies after plasmid transformation in Escherichia coli/E. coli.

Plasmid Maintenance: Routine propagation of kanamycin-marked plasmids in E. coli under 50 µg/mL kanamycin selection.

Colony Isolation and Purification: Streaking and single-colony purification on LB 1% glucose with kanamycin.

CFU Enumeration: Quantifying viable kanamycin-resistant cells via spread plating.

High-Density Library Plating: 150 mm plates for screening mutant/genomic libraries under kanamycin selection.

Replica Plating: Verification of kanamycin resistance and genotype confirmation across selective media.

Conjugation/Transposon Selections: Selecting transconjugants or insertions carrying kanamycin resistance in E. coli.

CRISPR/Cas Workflows: Selection of editing plasmids with kanamycin markers in E. coli.

Reduced Basal Expression: Using 1% glucose to suppress leaky expression from lac/T7–lac systems prior to induction.

Competent Cell QC: Transformation efficiency testing with kanamycin-resistance control plasmids.

Short-Term Archiving: Preparing working stocks and master plates of kanamycin-resistant strains.