Antibiotic Selection of Transformants: Isolation of carbenicillin-resistant Escherichia coli colonies carrying bla-based plasmids at 100 µg/mL carbenicillin.

Plasmid Maintenance and Clone Stabilization: Routine passaging of bla-selectable plasmids in E. coli, with carbenicillin providing stable beta-lactam selection.

Colony Screening and Picking: Growth of single colonies for downstream minipreps, PCR colony screening, and sequence verification.

Transformation Recovery Plating: Post-transformation plating to quantify CFUs and calculate transformation efficiency under beta-lactam selection.

Streaking for Isolation: Single-colony isolation from mixed cultures or glycerol stocks of E. coli to ensure clonal purity.

Library Plating: Selection and maintenance of plasmid libraries (e.g., mutagenesis or sgRNA libraries) requiring carbenicillin resistance.

Recombinant Construct Pre-screening: Screening of clones harboring constructs with catabolite-sensitive promoters, with 1% glucose helping reduce leaky expression during selection.

Maintenance of Assembly Products: Recovery and propagation of products from TA, Gateway, or Golden Gate cloning under carbenicillin selection.

Quality Control of Strains: Verification of antibiotic resistance markers and plasmid retention in E. coli strains.