Selection of Blasticidin-Resistant Transformants: Plating transformed E. coli to select colonies carrying plasmids with bsd/bsr resistance at 100 µg/mL blasticidin.

Plasmid Maintenance and Propagation: Routine streaking and passaging of recombinant E. coli strains to maintain blasticidin-selectable plasmids.

Colony Screening and Verification: Picking colonies for colony PCR, restriction analysis, or sequencing from blasticidin-selective plates.

CRISPR/Cas9 and Genome Editing Workflows: Selecting edited bacterial clones carrying blasticidin-marked editing or helper plasmids.

Protein Expression Clone Selection: Isolating expression strains with blasticidin-resistant expression vectors prior to small-scale or large-scale induction.

Library Construction and Clonal Isolation: Selecting and isolating clones from plasmid or mutagenesis libraries using blasticidin resistance in E. coli.

Antibiotic Susceptibility/Resistance Confirmation: Verifying functional blasticidin resistance markers in engineered bacterial strains.

Cross-Contamination Control in Mixed Cultures: Suppressing growth of non-resistant background microbes while recovering target blasticidin-resistant colonies.

Alternative Host Selection (as applicable): Selecting blasticidin-resistant transformants in other amenable bacteria (e.g., Bacillus subtilis) when a compatible bsd/bsr marker is present.