Selective Plating of Transformants: Recovery and selection of plasmid-bearing E. coli on 100 µg/mL carbenicillin after transformation.

Plasmid Maintenance and Propagation: Routine culture of beta-lactam–resistant E. coli strains to maintain plasmids with bla/ampR.

Colony Isolation and CFU Enumeration: Streaking for single colonies and counting viable cells on LB Lennox agar.

Cloning and Screening Workflows: Colony picking and insert verification; compatible with blue/white screening when supplemented with X-gal/IPTG.

Expression Construct Screening: Selecting and prescreening recombinant expression clones in E. coli prior to induction.

Glycerol Stock Revival: Reviving stored strains onto selective plates to ensure plasmid retention.

Conjugation/Mating Selections: Selecting transconjugants carrying beta-lactam–resistant plasmids on Gram-negative hosts.

Strain Verification and Stability Testing: Assessing plasmid stability and segregation under beta-lactam selection pressure.

General Gram-Negative Selection: Selection of susceptible non-enteric Gram-negatives (e.g., Pseudomonas aeruginosa) carrying carbenicillin-resistance markers.