Antibiotic Selection of Transformants: Selects colonies carrying tetracycline-resistance plasmids in Escherichia coli on LB Lennox (1.0% Tryptone, 0.5% Yeast Extract, 0.5% Sodium Chloride, 1.5% Agar; 12.5µg/mL Tetracycline).

Plasmid Maintenance and Amplification: Maintains selective pressure to preserve Tet^R plasmids during routine growth of Escherichia coli cultures and colony expansion.

Clone Screening: Enables screening of recombinant colonies carrying Tet^R backbones, including use with color or fluorescence reporters on compatible vectors.

Strain Construction and Genome Engineering: Supports selection of strains edited with Tet^R-linked markers or cassettes in Escherichia coli and related hosts.

Conjugation and Mating Selection: Recovers transconjugants bearing Tet^R markers after biparental or triparental matings (e.g., Escherichia coli, Agrobacterium tumefaciens).

Plasmid Stability and Segregation Assays: Assesses maintenance of Tet^R plasmids under selective conditions across passages.

Library and BAC/Fosmid Workflows: Selects and propagates Tet^R-based genomic libraries or large-insert clones in Escherichia coli.

Contamination Control in Mixed Cultures: Suppresses non-resistant background when enriching Tet^R strains such as Escherichia coli or Bacillus subtilis carrying compatible markers.