Plasmid Selection and Maintenance: Selects and maintains bla (β-lactamase)-encoded plasmids in E. coli at 100 µg/mL carbenicillin.

Transformation Recovery and Screening: Plates transformed E. coli to recover and select carbenicillin-resistant colonies.

Colony Isolation and Purification: Streaks single colonies to obtain clonal isolates from mixed or contaminated cultures.

Colony PCR Screening: Supports rapid screening of transformants by colony PCR directly from selected colonies.

CFU Enumeration: Determines colony-forming units of carbenicillin-resistant E. coli following transformation or conjugation.

Pre-expression Strain Verification: Streaks recombinant E. coli expression strains for plasmid confirmation prior to induction.

Conjugation/ Mating Selection: Selects transconjugants carrying bla-marked plasmids after bacterial mating experiments.

Blue-White Screening (with additives): Enables α-complementation when supplemented with X-gal/IPTG for insert screening in E. coli.

Clone Library Plating: Plates and picks libraries for downstream sequencing or functional screening under β-lactam selection.

Agrobacterium Plasmid Maintenance (as appropriate): Maintains bla-bearing vectors in Agrobacterium tumefaciens when carbenicillin selection is suitable.