Selection of Ampicillin-Resistant Transformants: Isolates colonies harboring AmpR (bla) plasmids after transformation of E. coli.

Plasmid Maintenance and Propagation: Maintains selective pressure to preserve bla-encoded plasmids during routine culture of E. coli.

Colony Screening (Colony PCR/Sanger Prep): Enables rapid picking of single colonies for colony PCR and downstream sequence verification.

Blue-White Screening: When supplemented with X-Gal and IPTG, supports α-complementation screening of recombinant clones on Amp plates.

Transformation Efficiency and CFU Enumeration: Quantifies transformation success by counting CFUs under ampicillin selection.

Cloning and Subcloning Workflows: Supports construction, recovery, and verification of recombinant plasmids requiring Amp selection.

Strain and Plasmid Archiving: Provides isolated colonies for establishing glycerol stocks and master seed lots.

Phenotypic Verification of AmpR Markers: Confirms presence and expression of β-lactamase-encoded resistance in transformed E. coli.

Starter Culture Initiation: Supplies single colonies for inoculating selective liquid media for plasmid prep or expression pre-cultures.

Contamination Control via Selection: Reduces growth of non-resistant background organisms, aiding clean recovery of target E. coli clones.