Selective Plating of bla-Encoded Plasmids: Enables antibiotic selection of beta-lactamase plasmids in E. coli using 200 µg/mL carbenicillin.

Bacterial Transformation and Colony Isolation: Used for post-transformation recovery and isolation of individual recombinant colonies in E. coli.

Plasmid Maintenance and Strain Purification: Supports streaking and passaging to maintain plasmids and obtain clonal isolates under beta-lactam selection.

Colony Forming Unit (CFU) Enumeration: Facilitates quantification of viable, plasmid-bearing cells under selective pressure.

Colony PCR Screening: Provides selective growth of transformants for downstream colony PCR verification of inserts.

Library Construction and Screening: Used to plate and screen recombinant DNA libraries while maintaining selection for bla-marked vectors.

Starter Culture Prep for Protein Expression: Enables picking verified colonies for selective overnight cultures prior to expression in E. coli.

Reduced Satellite Colonies vs. Ampicillin Plates: Carbenicillin helps minimize satellite colonies during selection relative to ampicillin.

Selection in Alternative Hosts: Applicable to other susceptible Gram-negative bacteria (e.g., Agrobacterium tumefaciens, Pseudomonas) carrying carbenicillin-resistant plasmids.

Conjugation/Transconjugant Selection: Used to select for transconjugants that acquire bla-bearing plasmids after mating experiments.