Selection of Kanamycin-Resistant Transformants: Isolates colonies carrying KanR plasmids in E. coli using LB (1.0% Tryptone, 0.5% Yeast Extract, 1.0% NaCl, 1.5% Agar) with 100µg/mL Kanamycin.

Cloning and Plasmid Maintenance: Propagates and maintains recombinant KanR plasmids; streaking and colony picking for stable constructs.

Colony Isolation and CFU Enumeration: Plates dilutions to count CFUs under kanamycin selection and obtain single colonies.

Screening of Genome Edits/Insertions: Selects for chromosomal integrations or edits conferring kanamycin resistance in E. coli.

Protein Expression Workflows: Starts cultures for KanR expression vectors in E. coli, ensuring antibiotic selection before induction.

Conjugation/Transposition Selection: Recovers recipients acquiring KanR markers after mating or transposition events.

Library and Mutant Pool Maintenance: Maintains KanR mutant libraries or barcoded pools on selective plates.

Glycerol Stock Preparation: Picks verified KanR colonies to establish long-term freezer stocks.