Antibiotic-Resistant Clone Selection: Selects transformants carrying kanamycin-resistance markers (e.g., nptII/kanR) in Escherichia coli using 30µg/mL kanamycin.

Plasmid Maintenance and Propagation: Maintains plasmids with kanamycin resistance in Escherichia coli and related bacteria (e.g., Salmonella enterica).

Colony Screening After Transformation: Enables identification of recombinant colonies on selective LB agar prior to colony PCR or sequencing.

CRISPR/Cas and Genome Editing Selection: Selects edited clones incorporating kanamycin resistance cassettes for verification and downstream workflows.

Transposon Mutagenesis Libraries: Recovers and maintains kanamycin-marked transposon insertion mutants for mapping and phenotype screens.

Bacterial Conjugation/Mating Selection: Selects transconjugants harboring kanamycin-marked plasmids or inserts while suppressing donor/recipient backgrounds.

Replica Plating and Strain Verification: Verifies genotype by transferring colonies onto kanamycin plates to confirm resistance-linked constructs.

Transformation Efficiency Quantification: Enumerates colony-forming units of kanamycin-resistant transformants to assess protocol performance.

Strain Maintenance and Streaking: Preserves selection pressure during routine streaking and short-term storage of kanamycin-bearing strains.

Synthetic Biology Circuit Development: Supports iterative design-build-test cycles for kanamycin-selectable plasmid systems in Escherichia coli.