Selection of Kanamycin-Resistant Clones: Isolates colonies carrying kanamycin-resistance markers (25 µg/mL) on LB agar (1.0% tryptone, 0.5% yeast extract, 1.0% NaCl, 1.5% agar).

Plasmid Maintenance: Maintains plasmids with kanamycin resistance in Escherichia coli to prevent plasmid loss during routine culturing.

Transformation Plating: Recovers and selects transformants after heat-shock or electroporation of E. coli with KanR plasmids.

Cloning Workflows: Selects correct assemblies after ligation, Gibson, or Golden Gate cloning using kanamycin-selectable vectors.

CFU Enumeration Under Selection: Quantifies viable kanamycin-resistant cells in mixed or experimental populations.

Conjugation/Mating Selections: Selects transconjugants carrying kanamycin markers in strains such as E. coli or Agrobacterium tumefaciens.

Genome Editing Selections: Screens recombinants after CRISPR or recombineering workflows using kanamycin cassettes.

Strain Preservation and Streaking: Streaks for single colonies and preserves kanamycin-selected strains on solid medium.

Quality Control of Resistance Markers: Verifies presence and expression of kanamycin resistance in bacterial stocks.

Phage or Library Screens: Maintains selection in phage display or pooled library experiments requiring KanR in E. coli.