Selection of Chloramphenicol-Resistant Transformants: Isolates colonies carrying chloramphenicol-selectable plasmids or genomic inserts in Escherichia coli.

Plasmid and BAC/PAC/Cosmid Maintenance: Propagates chloramphenicol-selectable vectors and libraries in Escherichia coli at 12.5 µg/mL.

Colony Screening and Picking: Enables selection-based cloning workflows (e.g., miniprep prep, Sanger prep) for chloramphenicol-marked constructs.

CFU Enumeration Under Selection: Quantifies viable chloramphenicol-resistant cells after transformation, conjugation, or transduction.

Replica Plating for Knockout Verification: Screens gene disruptions using cat (chloramphenicol acetyltransferase) cassettes in Escherichia coli.

Transposon Mutagenesis Selection: Recovers chloramphenicol-marked transposon insertions in Escherichia coli and Salmonella enterica.

CRISPR Editing Workflows: Selects edits that retain or integrate chloramphenicol resistance markers.

Conjugation and Mating Selections: Selects recipients or exconjugants acquiring chloramphenicol-marked plasmids.

Strain Maintenance Under Antibiotic Pressure: Maintains chloramphenicol-resistant lab strains of Escherichia coli and other bacteria (e.g., Bacillus subtilis).

High-Density Colony Arrays on 150 mm Plates: Supports large-scale screening and library plating with chloramphenicol selection.