Antibiotic Selection of Transformants: Isolates colonies carrying bla/ampR plasmids on 100 µg/mL carbenicillin LB agar, commonly in Escherichia coli.

Clonal Isolation (Streaking for Singles): Generates single, genetically uniform colonies from mixed or transformed cultures.

Colony PCR Screening: Provides colonies as templates to verify inserts or constructs directly from carbenicillin-selected plates.

CFU Enumeration and Viability: Quantifies transformation efficiency and cell viability under beta-lactam selection.

Plasmid Maintenance and Stability Testing: Maintains selection pressure to assess plasmid retention across passages.

Strain Recovery from Stocks: Revives and amplifies stored carbenicillin-resistant strains (e.g., Escherichia coli) for downstream culture.

Pre-culture Plate Setup for Cloning/Expression: Prepares verified colonies for inoculation into liquid media with beta-lactam selection.

Conjugation/Mating Selections: Selects transconjugants carrying beta-lactam–resistant plasmids after biparental or triparental matings.

Host Range and Susceptibility Workflows: Plates carbenicillin-resistant Gram-negative hosts (e.g., Pseudomonas aeruginosa) to assess construct portability.

Contamination Control on Plates: Suppresses non-resistant contaminants while propagating target resistant strains.