Antibiotic Selection of Transformants: Selects E. coli colonies carrying plasmids with bla (amp/carbenicillin) resistance.

Plasmid Cloning and Maintenance: Maintains plasmid-bearing E. coli strains under carbenicillin selection pressure.

Colony Screening (e.g., Colony PCR): Enables rapid screening of antibiotic-resistant colonies for correct inserts.

Expression Strain Screening: Plates recombinant E. coli expressing proteins from carbenicillin-selectable vectors.

Library Construction and Preservation: Supports construction and maintenance of carbenicillin-selectable genomic/cDNA libraries in E. coli.

Blue–White Screening (with Supplements): Performs α-complementation screens on LB + X-gal/IPTG with carbenicillin-selectable plasmids.

Conjugation/Mating Selections: Selects recipients or transconjugants carrying bla-marked plasmids in E. coli.

CFU Enumeration under Selection: Quantifies viable antibiotic-resistant E. coli after transformations or stress assays.

Strain Preservation Workflows: Picks isolated colonies for glycerol stocks or master plates while maintaining selection.

Quality Control of Plasmid Stability: Monitors retention of bla-marked plasmids in cultured E. coli populations.