Selection of Ampicillin-Resistant Transformants: Isolates colonies of Escherichia coli carrying AmpR plasmids after transformation.

Plasmid Maintenance and Expansion: Maintains selective pressure for AmpR plasmids during streaking, colony picking, and overnight growth of E. coli.

Colony Screening (Colony PCR/Sanger Prep): Enables picking AmpR colonies of E. coli for rapid insert verification and sequencing.

Blue-White Screening: Supports alpha-complementation on Amp plates for cloning when supplemented with X-gal/IPTG in E. coli.

Transformation Efficiency and CFU Enumeration: Quantifies viable Amp-resistant E. coli colonies after ligation or Gibson assembly.

Protein Expression Workflow Setup: Selects AmpR expression strains (e.g., T7-based) of E. coli prior to induction in liquid culture.

Replica Plating and Plasmid Stability Tests: Assesses retention of AmpR plasmids in E. coli under selective pressure.

Conjugation/Cloning Workflows: Selects AmpR recipients or donors post-mating or recombineering steps in E. coli.

Glycerol Stock Recovery: Recovers and verifies AmpR E. coli strains from frozen stocks before experiments.

Educational and Teaching Labs: Demonstrates antibiotic selection and basic cloning using AmpR E. coli on solid media.