Antibiotic Selection of Transformants: Selects and maintains plasmid-bearing, ampicillin-resistant Escherichia coli clones after transformation.

Colony Isolation and Picking: Streaks or spreads to obtain single Amp^R colonies of Escherichia coli for downstream cloning and analysis.

Colony Screening for Recombinants: Screens transformed Escherichia coli colonies (e.g., PCR or reporter assays) on selective LB–Amp (50 µg/mL) plates.

Plasmid Maintenance and Stability Testing: Maintains and assesses stability of Amp^R plasmids in Escherichia coli under selection.

CFU Enumeration under Selection: Quantifies transformation efficiency by counting colony-forming units of Amp^R Escherichia coli.

Library Construction and Archiving: Builds and preserves cDNA/genomic or sgRNA libraries carried on Amp^R plasmids in Escherichia coli.

Replica Plating and Counter-Selection Workflows: Replicates Amp-selected colonies for comparative screening or phenotype verification in Escherichia coli.

Reporter and Expression Construct Screening: Evaluates expression or reporter activity from Amp^R plasmids in Escherichia coli on selective plates.

High-Density Plating on Large Format: Uses 150 mm plates for high-throughput colony arrays, colony lifts, or pooled screens with Amp selection in Escherichia coli.

Conjugation/Plasmid Transfer Selection: Selects recipients acquiring Amp^R plasmids after mating or transduction in Escherichia coli.